Lisheng Wang

Lisheng Wang

Associate Professor 

Department of Biochemistry, Microbiology, and Immunology

Degrees:

Ph.D., University of Sydney (1999)

Contact info:

Office: Room 4104, Guindon Hall
Phone:

(613) 562-5624 (Office); 
(613) 562-5800 x8371 (Lab)

Fax: (613) 562-5452
Email: lwang@uottawa.ca

Biographical Sketch:

Dr. Lisheng WANG is an Associate Professor in the Department of Biochemistry, Microbiology, and Immunology at University of Ottawa, a Core Member at the Ottawa Institute of Systems Biology, and an Affiliated Investigator at the Ottawa Health Research Institute. He graduated from Medical School in China and received his clinical training as a Fellow in Australian National Liver Transplantation Unit. Dr. Wang completed a PhD at the University of Sydney (Australia) studying the transplantation immunology. He then pursued postdoctoral research in immunology at Harvard Medical School, USA, and further postdoctoral research in stem cell biology and regenerative medicine at Robarts Research Institute, Canada.

Research Interests:

Using cellular and molecular approaches and in vivo animal models, Dr. Wang’s laboratory is interested in: 1) nurturing human embryonic stem cells (hESCs) into mothers of circulatory cells and immune cells, generating transplantable cells for stem cell-based clinic therapy and reducing transplantation rejection; 2) targeting embryonic signaling pathways in cancer for more effective treatment.

hESCs can theoretically divide without limit (termed self-renewal) and grow into almost any type of cell (termed pluripotency), raising attractive prospect of cell replacement therapy to treat or even cure many diseases. Maintenance and differentiation of hESCs are challenging. Using rigorous criteria, Dr. Wang’s laboratory has established and improved the techniques to expand, freeze-thaw, maintain, evaluate and differentiate hESCs. Dr. Wang and his colleagues have defined mother cells of endothelium (lining cells in circulatory vessels) and blood during differentiation of hESCs. These mother cells can give rise to either endothelium or blood under different biochemical signals. Further studies have shown that hESC-derived blood cells have repopulating capacity in immunodeficient mice (blood stem cell property). These findings have set up a foundation for future studies.

Note: Dr. Wang is actively recruiting graduate students, postdoctoral fellows and research staff for his lab.

For further information, please contact Dr. Lisheng Wang at 613-562-5624 or lwang@uottawa.ca

Selected Publications:

  • Tian R#, Wang S# (#equal contribution), Elisma F, Li L, Zhou H, Wang L*, Figeys D* (*correspondence authors). Rare Cell Proteomic Reactor Applied to Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-based Quantitative Proteomics Study of Human Embryonic Stem Cell Differentiation. Mol Cell Proteomics. 2011 Feb;10(2):M110.000679. Epub 2010 Jun 8
  • Mohib K, Allan D, Wang L. Human embryonic stem cell-extracts inhibit the differentiation and function of monocyte-derived dendritic cells. (Original research article) Stem Cell Rev. 2010 Dec;6(4):611-21
  • Li L#, Wang S#, Jezierski A# (# equal contribution), Moalim-Nour L, Mohib K, Parks RJ, Francesco Retta S, Wang L. A unique interplay between Rap1 and E-cadherin in the endocytic pathway regulates self-renewal of human embryonic stem cells. Stem Cells2010; 28: 247-257 (Top downloaded articles in Stem Cells in February 2010)
  • Li L, Wang BH, Wang S, Moalim-Nour L, Mohib K, Lohnes D, Wang L.Individual cell movement, asymmetric colony expansion, Rho-associated kinase and E-cadherin impact the clonogenic assay of human embryonic stem cells. Biophysical Journal2010; 98: 2442-2551 (Featured article).
  • Zhong S, Magnolo AL, Sundaram M, Zhou H, Yao EF, Leo ED, Loria P, Wang S, Bamji-Mirza M, Wang L, McKnight J, Figeys D, Wang Y, Tarugi P, and Yao Z. Nonsynonymous mutations within APOB in human hypobetalipoproteinemia: Evidence for feedback inhibition of lipogenesis and post-endoplasmic reticulum degradation of apolipoprotein B. J Biol Chem  2009; Dec 23: PMID: 20032471 (Epub ahead of print).
  • Jezierski A, Swedani A, Wang L. Development of Hematopoietic and Endothelial Cells from Human Embryonic Stem Cells: Lessons from the Studies using Mouse as a Model. TheScientificWorldJOURNAL 2007; 7: 1950-1964 (Review).
  • Wang L. Endothelial and hematopoietic cell fate of human embryonic stem cells. Trends in Cardiovascular Medicine. 2006; 16:89-94.
  • Menendez P, Bueno C, Wang L. Human embryonic stem cells: A journey beyond cell replacement therapies. Cytotherapy. 2006; 8(6):530-41.
  • Cerdan C, Bendall SC, Wang L, Stewart M, Werbowetski T, Bhatia M. Complement targeting of nonhuman sialic acid does not mediate cell death of human ESCs. Nature Medicine 2006; 12: 1113-1114.
  • Wang L*, Menendé P* (*equal contribution), Shojaei F, Li L, Mazurier F, Dick JE, Cerdan C, Levac K, Bhatia M. Generation of hematopoietic repopulating cells from human embryonic stem cells independent of ectopic HoxB4 expression. Journal of Experimental Medicine 2005; 201: 1603-1614
  • Wang L, Li L, Menendez P, Cerdan C, Goodale D, Bhatia M. Human embryonic stem cells maintained in the absence of mouse embryonic fibroblasts or conditioned media are capable of hematopoietic development. Blood 2005; 105: 4598-4603.
  • Roura-Mir C*, Wang L* (* equal contribution), Cheng TC, Matsunaga I, Dascher C, Peng S, Fenton MJ, Moody DB. M. tuberculosis regulates CD1 antigen presentation pathways through TLR-2. Journal of Immunology2005; 175:1758-1766.
  • Wang L, Menendez P, Cerdan C, Bhatia M. Hematopoietic cells from human embryonic stem cells. Experimental Hematology (Review) 2005; 33:987-96
  • Menendez P, Bueno C, Wang L, Bhatia M. Human Embryonic Stem Cells: Potential tool towards achieving immunotolerance? Stem Cell Reviews 2005; 1: 151-158..
  • Menendez P, Wang L, Bhatia M. Genetic manipulation of human embryonic stem cells: a powerful approach for studies in human development and potential therapeutic applications. Current Gene Therapy (Review) 2005; 5: 375-85.
  • Wang L, Li L, Shojaei F, Levac K,Cerdan C,Menendez P, Martin T, Rouleau A, Bhatia M.Endothelial and hematopoietic cell fate of human embryonic stem cells originates from primitive endothelium with hemangioblastic properties. Immunity 2004; 21: 31-41.
  • Menendez P, Wang L, Chadwick K, Li L & Mickie Bhatia. Retroviral transduction of hematopoietic cells differentiated from human embryonic stem cell-derived CD45 negPFV hemogenic precursors. Molecular Therapy 2004; 10: 1109-1120.
  • Chadwick K*, Wang L* (*equal contribution), Li L, Menendez P, Murdoch B, Rouleau A, Bhatia M. Cytokines and BMP-4 promote hematopoietic differentiation of human embryonic stem cells. Blood 2003; 102: 906-915. (Comment) in Blood 2003; 102: 776.
  • Kamath A, Wang L, Das H, Lin L, Reinhold VN, Bukowski JF. Tea Drinking Enhances Immunity to Microbial Antigens by Priming gd T Cells in vivo. Proc. Natl. Acad. Sci. U. S. A.2003; 100: 6009-6014.
  • Wang L, Das H, Kamath A, Lin L, Bukowski JF. Human V g2V d2 T cells augment Migration-inhibitory factor secretion, and counteract the inhibitory effect of glucocorticoids on IL1- b and TNF- a production. Journal of Immunology 2002; 168: 4889-4896.
  • Wang L, Kamath A, Das H, Lin L, Bukowski JF. Antibacterial effect of human V g2V d2 T cells in vivo. Journal of Clinical Investigation 2001; 108: 1349-1357.
  • Wang L, Das H, Kamath A, Bukowski JF. Human V g2V d2 T cells produce IFN- g and TNF- a with an on/off/on cycling pattern in response to live bacterial products. Journal of Immunology 2001; 167: 6195-6201.
  • Das H, Wang L, Kamath A and Bukowski JF. V g 2 d 2 T cell receptor–mediated recognition of aminobisphosphonate. Blood2001; 98(5): 1616-1618.
© University of Ottawa
For additional information, consult our list of contacts
Technical questions? medtech@uottawa.ca
Last updated: 2012.01.12